28th March 2010
| Final | Carlisle United | 1-4 | Southampton |
9th February 2010
| Southern Section Final 2nd Leg | Southampton | 3-1 | Milton Keynes Dons |
| Northern Section Final 2nd Leg | Carlisle United | 2-3 | Leeds United |
20th January 2010
| Southern Section Final 1st Leg | Milton Keynes Dons | 0-1 | Southampton |
19th January 2010
| Northern Section Final 1st Leg | Leeds United | 1-2 | Carlisle United |
15th December 2009
| Northern Section Semi-Final | Carlisle United | 3-0 | Bradford City |
| Southern Section Semi-Final | Southampton | 2-2 | Norwich City |
| Southern Section Semi-Final | 1-4 | Milton Keynes Dons |
| Northern Section Semi-Final | Leeds United | 2-0 | Accrington Stanley |
11th November 2009
| Southern Section Quarter-Final | Southampton | 2-1 | Charlton Athletic |
10th November 2009
| Northern Section Quarter-Final | Leeds United | 3-1 | Grimsby Town |
| Northern Section Quarter-Final | Bradford City | 2-2 | Port Vale |
| Northern Section Quarter-Final | Chesterfield | 1-3 | Carlisle United |
| Southern Section Quarter-Final | Leyton Orient | 1-1 |
| Southern Section Quarter-Final | Swindon Town | 0-0 | Norwich City |
| Northern Section Quarter-Final | Accrington Stanley | 3-2 | Bury |
| Southern Section Quarter-Final | Milton Keynes Dons | 3-1 | Northampton Town |
20th October 2009
| Northern Section 2nd Round | Accrington Stanley | 2-0 | Shrewsbury Town |
6th October 2009
| Southern Section 2nd Round | Southampton | 2-2 |
| Southern Section 2nd Round | 2-2 | Aldershot Town |
| Southern Section 2nd Round | Milton Keynes Dons | 2-0 | Southend United |
| Southern Section 2nd Round | Leyton Orient | 1-0 | Brighton & Hove Albion |
| Southern Section 2nd Round | Charlton Athletic | 4-1 | Barnet |
| Southern Section 2nd Round | Gillingham | 0-1 | Norwich City |
| Southern Section 2nd Round | Northampton Town | 2-1 | AFC Bournemouth |
| Southern Section 2nd Round | Exeter City | 1-1 | Swindon Town |
| Northern Section 2nd Round | Hartlepool United | 0-2 | Grimsby Town |
| Northern Section 2nd Round | Bury | 2-1 | Tranmere Rovers |
| Northern Section 2nd Round | Carlisle United | 4-2 | Macclesfield Town |
| Northern Section 2nd Round | Port Vale | 3-1 |
| Northern Section 2nd Round | Bradford City | 2-2 |
| Northern Section 2nd Round | Leeds United | 2-1 |
| Northern Section 2nd Round | Chesterfield | 3-3 | Huddersfield Town |
1st September 2009
| Northern Section 1st Round | Rochdale | 1-2 | Bradford City |
| Northern Section 1st Round | Rotherham United | 1-2 | Huddersfield Town |
| Northern Section 1st Round | Oldham Athletic | 1-2 | Accrington Stanley |
| Northern Section 1st Round | Morecambe | 2-2 | Carlisle United |
| Northern Section 1st Round | 1-0 | Lincoln City |
| Northern Section 1st Round | Crewe Alexandra | 1-4 |
| Northern Section 1st Round | Walsall | 0-0 | Bury |
| Southern Section 1st Round | Barnet | 2-0 | Millwall |
| Southern Section 1st Round | AFC Bournemouth | 2-1 |
| Southern Section 1st Round | Cheltenham Town | 1-3 |
| Southern Section 1st Round | Gillingham | 1-1 | Colchester United |
| Southern Section 1st Round | 0-0 | Bristol Rovers |
| Southern Section 1st Round | Milton Keynes Dons | 3-1 | Dagenham & Redbridge |
| Southern Section 1st Round | Norwich City | 1-0 | Brentford |
| Southern Section 1st Round | Wycombe Wanderers | 2-2 | Northampton Town |
| Northern Section 1st Round | Burton Albion | 1-5 | Chesterfield |